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Characterization Of A New ODA3 Allele, ODA3-6, Defective In Assembly Of The Outer Dynein Arm-Docking Complex In Chlamydomonas Reinhardtii
Mosley, Matthew Brown, Jason Montes-Berrueta, Daniela Mosley, Matthew Hou, Yuqing Montes-Berrueta, Daniela Yang, Fan Hou, Yuqing Scarbrough, Chasity Yang, Fan
Mosley, Matthew
Brown, Jason
Montes-Berrueta, Daniela
Mosley, Matthew
Hou, Yuqing
Montes-Berrueta, Daniela
Yang, Fan
Hou, Yuqing
Scarbrough, Chasity
Yang, Fan
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Title
Characterization Of A New ODA3 Allele, ODA3-6, Defective In Assembly Of The Outer Dynein Arm-Docking Complex In Chlamydomonas Reinhardtii
Date
2017-03-14
Subject
5' UTR
Chlamydomonas reinhardtii
dyneins
flagella
phenotypes
plasmid construction
polymerase chain reaction
swimming
Chlamydomonas reinhardtii
dyneins
flagella
phenotypes
plasmid construction
polymerase chain reaction
swimming
Material type
Collections
Abstract
We have used an insertional mutagenesis approach to generate new C. reinhardtii motility mutants. Of 56 mutants isolated, one is a new allele at the ODA3 locus, called oda3-6. Similar to the previously characterized oda3 alleles, oda3-6 has a slow-jerky swimming phenotype and reduced swimming speed. The oda3-6 mutant fails to assemble the outer dynein arm motor and outer dynein arm—docking complex (ODA-DC) in the ciliary axoneme due to an insertion in the 5’ end of the DCC1 gene, which encodes the DC1 subunit of the ODA-DC. Transformation of oda3-6 with the wild-type DCC1 gene rescues the mutant swimming phenotype and restores assembly of the ODA-DC and the outer dynein arm in the cilium. This is the first oda3 mutant to be characterized at the molecular level and is likely to be very useful for further analysis of DC1 function.
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PLoS ONE 12(3): e0173842. doi:10.1371/journal.pone.0173842